Frequently Asked Questions
Everything we get asked, organized into nine plain-English categories — from how the 21+ attestation works to how your COA gets generated. Can’t find what you’re looking for? Contact support and a person will answer.
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ISO 9001 and ISO/IEC 17025 answer two different questions. ISO 9001 asks whether an organization runs a controlled, documented quality-management system. ISO/IEC 17025 asks whether a testing laboratory is technically competent to produce valid results. Peptriva holds both.
The distinction matters when you read a certificate. A quality-management system governs records, change control and how deviations are handled. It says nothing about whether an HPLC result is correct. Laboratory competence covers method validation, equipment calibration, analyst training and measurement uncertainty. That is what makes a number defensible.
Neither standard is a result. A lot is only described by its own data. On the independent certificate issued per lot by an independent US laboratory, currently Freedom Diagnostics in Franklin TN, identity is run by LC-MS and reports Confirmed. Purity is HPLC-UV, area-percent, with real per-lot values from 99.58% to 99.91%. Net content is measured in mg and often sits above the label. Endotoxin uses the LAL assay in accordance with USP <85>, run in duplicate, with both replicates reporting Pass. Heavy metals are tested and reported within the purity panel.
Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. Release is enforced in code: a lot cannot move into sellable stock unless both the manufacturing-facility certificate and the independent certificate are on file.
All products are research use only, not for human or veterinary use.
A lot is one finished production run of one compound. Lot-level testing means the analysis is run on that lot, and the numbers on the certificate belong to that lot alone. Batch sampling is different. It tests a subset and treats the result as representative of everything around it. That is a statistical argument. Lot-level testing is a direct measurement.
The independent laboratory certificate reports identity, purity, net content and endotoxin for each lot, and heavy metals are reported within the purity panel. Identity by LC-MS, reported as Confirmed. Purity by HPLC-UV, in area-%. Real lots have come back between 99.58% and 99.91%. Net content, measured in mg. Endotoxin by LAL assay, in accordance with USP <85>, run in duplicate, with both replicates reporting Pass. Heavy metals are tested and reported within the purity panel.
Net content shows why this matters. BPC-157 lot BC10-260504 reported 11.24 mg against a 10 mg label. A sample from a different lot would not have told you that.
Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. Release is enforced in code. poReadyToSync() refuses to move a lot into sellable stock unless both the manufacturing facility certificate and the independent certificate are on file.
The limits are worth stating plainly. A certificate describes its own lot and nothing else. Microbial analysis by PCR appears on the bacteriostatic water certificate, and is not a per-lot peptide test. Everything is research use only, not for human or veterinary use.
Purity and net content answer different questions. Purity is a ratio. HPLC-UV area-% tells you what share of the material is the target peptide. It does not tell you how much material is there. A lot can pass purity and still be short of the label weight. Net content closes that gap. It is the measured mass, in milligrams, of what the vial holds.
That matters because you cannot check it yourself. Nothing about the vial shows its mass. Without a measured figure you are trusting a printed number. With one you are reading a result.
Peptriva's independent certificate prints net content in mg for every lot. Measured values frequently sit above the label. BPC-157 lot BC10-260504 reported 11.24 mg against a 10 mg label. Overfill of that kind is the direction you want the error to run.
One limit, stated plainly: net content is a per-lot measurement. It describes the lot, not the single vial in your hand. The certificate does not claim otherwise.
The release gate here is code, not policy. poReadyToSync() will not move a lot into sellable stock unless both the manufacturing facility certificate and the independent certificate are on file. The gate keys on the certificates themselves: without both certificates on file, the lot does not become sellable stock. Every product is research use only, not for human or veterinary use.
LC-MS answers one question: is this the molecule named on the label? HPLC-UV answers another: how much of what is in the vial is that molecule? Neither test substitutes for the other.
LC-MS separates the sample, then weighs its components by mass-to-charge ratio. A peptide has a known molecular mass. If the measured mass matches, identity is confirmed. The result is a word, not a percentage. The independent certificate prints identity as Confirmed.
HPLC-UV separates the sample on a column and detects each component by ultraviolet absorbance. The report is area-%: the main peak divided by the total peak area. Real per-lot values on our certificates run 99.58% to 99.91%. Heavy metals are tested and reported within the same purity panel.
Each test has a blind spot. Area-% only counts what the UV detector sees at that wavelength. Salts, water and residual solvents may absorb weakly or not at all, so area-% is not a mass fraction of the powder. That is why net content is measured separately, in mg per lot. Purity also says nothing about identity. A closely related peptide can run as a single clean peak and still be the wrong compound. LC-MS is what catches that.
Both tests appear on every lot certificate. Release is enforced in code: poReadyToSync() will not move a lot into sellable stock unless the manufacturing facility certificate and the independent laboratory certificate are both on file. Research use only. Not for human or veterinary use.
Heavy metals are screened because trace metals can enter a peptide at several points. Synthesis, purification, solvents, and storage contact all provide routes. The residue is usually small. It still matters in a research setting.
Metal ions are reactive. They can catalyze oxidation of sensitive residues such as methionine and cysteine, which changes the material over time. Some metals bind peptides directly and alter how they behave in an assay. In cell work, trace metal carryover can suppress viability and shift readouts. A result then gets blamed on the peptide when the contaminant caused it. Screening removes that ambiguity before the material reaches an experiment.
Peptriva tests heavy metals and reports them within the purity panel on the independent laboratory certificate. That certificate is issued per lot by an independent US laboratory, currently Freedom Diagnostics of Franklin, Tennessee. The same panel prints purity by HPLC-UV, with real per-lot values running from 99.58% to 99.91%. Identity is confirmed separately by LC-MS.
Two things are not on this page. We do not publish a numeric heavy-metals limit, and we do not name the instrument method used. Read the certificate for your own lot for what was actually measured.
Release is enforced in code, not policy. poReadyToSync() refuses to move a lot into sellable stock unless both the manufacturing facility certificate and the independent certificate are on file. Everything is research use only, not for human or veterinary use.
Endotoxin is lipopolysaccharide from the outer membrane of gram-negative bacteria. It is not a living organism. It is a molecule, and it is stable enough that it can outlast the bacteria that produced it and survive ordinary purification steps.
That is why a lab cares. Endotoxin is biologically active at extremely low concentrations. In cell culture it can activate immune-competent cells, shift cytokine readouts, and change proliferation results. A peptide that is chemically clean can still carry enough endotoxin to confound an assay, so a purity number alone does not answer the question.
The standard method is the Limulus amebocyte lysate assay, usually written LAL. USP <85> covers how the test is set up, controlled and interpreted. Peptriva's independent laboratory certificate reports LAL in accordance with USP <85>, run in duplicate, and both replicates report Pass. The certificate also prints an assay sensitivity of 0.05 EU/mL. That figure is the sensitivity of the assay itself. It is not a limit, not a specification and not the measured result for the lot.
Release is gated in code, not policy. A lot cannot move into sellable stock until both the manufacturing facility certificate and the independent certificate are on file.
Two limits worth stating plainly. An endotoxin pass says nothing about viable organisms; it is a test for a molecule, not a count of what is alive. And microbial analysis by PCR appears on our bacteriostatic water certificate only, not as a per-lot peptide test. Everything is research use only, not for human or veterinary use.
Ask which lot the document covers. A certificate with no lot number cannot be matched to the vial in your hand. Then ask who ran the test. In-house and independent certificates are different evidence. Ask for both.
Ask for the method on every line. Identity should be LC-MS. Purity should be HPLC-UV reported as area-%. A purity number with no method behind it means little. Ask for measured net content in mg, not the label weight. Measured content can sit above the label. One Peptriva lot reported 11.24 mg against a 10 mg label.
Ask how endotoxin was run. USP <85> covers the LAL assay. Peptriva's independent certificate runs it in duplicate, and both replicates report Pass. The certificate also prints an assay sensitivity of 0.05 EU/mL. That is what the assay can detect. It is not a limit and not a result. A supplier quoting it as a limit is misreading their own paperwork.
Ask what is not tested. Microbial PCR analysis appears on the bacteriostatic water certificate, not on each peptide lot. Ask which accreditation is held and what it means. ISO 9001 certifies a quality-management system. ISO/IEC 17025 concerns testing-laboratory competence. Peptriva holds both. Heavy metals are tested and reported within the purity panel.
Last, ask what stops an untested lot from shipping. At Peptriva that gate is code, not policy: poReadyToSync() refuses to move a lot into sellable stock unless both the manufacturing-facility certificate and the independent certificate are on file. All material is research use only, not for human or veterinary use.
A certificate of analysis covers one lot, not a product line. Four results carry the weight.
Identity is run by LC-MS and reports "Confirmed". Purity is HPLC-UV, reported as area-%. Recent lots print 99.58% to 99.91%. Area-% is relative. It tells you what share of detected material is the peptide, not an absolute mass. Heavy metals are tested and reported within that purity panel.
Net content is measured in mg for the lot, and it often lands above the label. BPC-157 lot BC10-260504 reported 11.24 mg against a 10 mg label. Endotoxin is the LAL assay in accordance with USP <85>, run in duplicate, and both replicates report "Pass". The certificate also prints an assay sensitivity of 0.05 EU/mL. That figure is the assay's detection sensitivity, not a limit, not a specification, and not a result.
Read what is missing too. Microbial analysis by PCR appears on the bacteriostatic water certificate. It is not a per-lot peptide test. A certificate describes the lot as tested, and nothing after that.
Peptriva files two certificates per lot. Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. The independent laboratory is currently Freedom Diagnostics, Franklin TN. Release is gated in code: poReadyToSync() refuses to move a lot into sellable stock unless both the manufacturing-facility certificate and the independent certificate are on file. Everything is research use only, not for human or veterinary use.
Check whether every result is a measurement. A real certificate prints a method, a lot number, a date, and a value you can check. A printed document just restates the label.
Read the identity line first. It should name a method. LC-MS is the usual one, and the result reads "Confirmed". Purity should name HPLC-UV and report area-%. Peptriva's per-lot certificates from an independent US laboratory run 99.58% to 99.91%.
Then check net content. A document that repeats the label size measured nothing. A real one reports the measured net content of the vial in mg. One BPC-157 lot, BC10-260504, reported 11.24 mg against a 10 mg label.
Endotoxin is where documents get vague. The certificate should name the assay and the standard. LAL in accordance with USP <85>, run in duplicate, both replicates "Pass". The certificate also prints an assay sensitivity of 0.05 EU/mL. That is what the assay can detect, not a limit and not a result. Treat any vendor quoting it as a specification with suspicion.
Ask what is not there. Heavy metals are tested and reported within the purity panel. Microbial analysis by PCR appears on the bacteriostatic water certificate, not on peptide lots.
Last, ask who checks. At Peptriva a lot cannot move into sellable stock until both the manufacturing facility certificate and the independent certificate are on file. That check is code, not policy. Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. Research use only, not for human or veterinary use.
The difference is who signs the result and who has something to lose. A self-reported claim is generated by the seller, or by the manufacturing facility that made the lot. It may be accurate, but nothing outside the seller confirms it. An independent third-party certificate comes from a laboratory that did not make the material and does not sell it.
For a scientist, the useful difference is method and traceability. It names the method, ties the result to a lot code, and reports a number rather than a claim. On Peptriva's independent certificate, identity is LC-MS and reads Confirmed. Purity is HPLC-UV, area-%, with real per-lot values between 99.58% and 99.91%. Net content is measured in mg, so it can disagree with the label. BPC-157 lot BC10-260504 reported 11.24 mg against a 10 mg label. Endotoxin runs by LAL assay in accordance with USP <85>, in duplicate, and both replicates report Pass. The certificate also prints an assay sensitivity of 0.05 EU/mL. That is the assay's detection sensitivity, not a limit and not a result. Heavy metals are tested and reported within the purity panel.
Peptriva enforces this in code. A release function, poReadyToSync(), refuses to move a lot into sellable stock unless both the manufacturing-facility certificate and the independent certificate are on file. Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center.
A certificate covers the lot it names, not every vial. All material is research use only, not for human or veterinary use.
Verification is a matching exercise before it is a reading exercise. The order matters, because every result on a certificate is only meaningful once you know it describes the vial you are holding.
1. Find the lot code on the vial. It is printed on the label, and it is the only field that ties the glass to a document. 2. Open that lot's certificate. The QR code on the vial resolves to the results for that lot. The same certificate is retrievable by entering the lot code at /coa, and past orders stay retrievable by lot number. 3. Compare the lot codes. Character for character, on the certificate against the vial. 4. Read the four results. Identity reported as Confirmed by LC-MS, purity as an HPLC-UV area-%, net content as a measured mass in mg, and endotoxin by the LAL assay in accordance with USP <85>, run in duplicate and reported as Pass.
Three cases are worth naming, because they are the ones that decide whether you have evidence at all. A certificate carrying no lot code is not a certificate for your vial, and no amount of detail elsewhere on it changes that. A certificate whose lot code differs from the vial describes a different batch, and should be treated as the wrong document rather than a close-enough one. A lot code on the vial that is illegible leaves you unable to verify anything, so ask rather than assume — contact us with the order and we will identify the lot.
The reason this check is cheap here is structural. A lot cannot be moved into sellable stock until both of its certificates are on file, the manufacturing facility's and the independent laboratory's, and that is enforced in code rather than by policy. What the certificate reports, field by field, is covered in the FAQ.
This is a release gate rather than a policy, and the distinction matters: held stock is not marked as unavailable in inventory, it is kept outside sellable inventory entirely, on the purchase order it arrived against. There is no state in which it can be sold by mistake.
Four cases are covered, and only one of them releases stock. Manufacturing facility's certificate only, which is what arrives with the shipment, stays held. Independent certificate only, without the manufacturer's, is not sellable. A lot received with no certificate at all is not sellable. A lot code that is not recognised is not sellable. Both certificates present is the only combination that releases the lot into sellable inventory.
That behaviour is covered by unit tests rather than by a written procedure, which is what is meant elsewhere on this site by the gate being enforced in code. If you believe a vial you received does not match its certificate, contact us with the lot code and we will take it from there.
Two certificates exist per lot and they are separated by the shipping leg. The manufacturing facility tests the finished lot, and across the seven lots where both dates are on file that certificate is dated 8 to 16 days after the lot date. The independent laboratory re-tests the same lot when it arrives at the cold-chain center, and across the eleven lots with a readable lot date that certificate was reported 36 to 65 days after the lot date.
Read those as a description of what has happened, not a promise about what will. The release rule is not time-based at all: a lot becomes sellable when both certificates are on file, and a slow re-test delays the release rather than shortening the testing. Every certificate currently on file is listed at /coa with its lot code, so the interval on any specific lot is checkable rather than taken on trust.
What is published is the independent laboratory's certificate itself, as the laboratory issued it, not a summary of it. It carries the lot code, the accession number, identity, purity, net content and the endotoxin result, and it is the document the figures on this site are drawn from.
What is not published is the underlying instrument output. There are no chromatograms or spectra behind the certificate on this site, and the manufacturing facility's own certificate, while required before a lot can be released, is held on file rather than shown. If your work needs something beyond the published certificate, ask with the lot code rather than assuming either way.
Yes. The independent certificate for every lot is issued by an independent US laboratory, currently Freedom Diagnostics in Franklin, Tennessee. That certificate prints identity by LC-MS, purity by HPLC-UV, net content in mg, and an endotoxin result run in duplicate under USP <85>.
Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. A lot cannot enter sellable stock until both the manufacturing-facility certificate and the independent certificate are on file. That check is enforced in our code, not just in policy. Peptriva holds ISO/IEC 17025 and ISO 9001.
Two things we do not say. We do not publish where the manufacturing facility runs its own testing. And US testing is not a statement about where the material is made.
Research use only. Not for human or veterinary use.
Orders ship FedEx 2-Day with cold-chain handling. Shipping is free on every order to the continental US.
FedEx 2-Day shipments move under a FedEx tracking number, so the parcel is tracked in transit. We do not publish an order cut-off time, and we do not promise same-day dispatch. The two-day clock is FedEx's service clock. It starts when the carrier takes the parcel, not when you place the order. If a delivery window matters to you, plan around that.
Not confirmed: standing orders, recurring shipments, and lab account pricing. We have no published program for any of these. Do not assume one exists until Peptriva says so.
All products are research use only. Not for human or veterinary use.
Peptriva is a research-peptide supplier. What makes it different is enforced in code, not policy: a lot cannot enter sellable stock unless two certificates are on file, one from the manufacturing facility and one from an independent US laboratory.
Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. The independent certificate prints identity by LC-MS and purity by HPLC-UV, with real lot values between 99.58% and 99.91%. It also prints measured net content in mg and an endotoxin result by LAL under USP <85>, run in duplicate. Heavy metals are tested within the purity panel. Peptriva holds ISO 9001 and ISO/IEC 17025.
Every lot is held in cold-chain handling from the manufacturing facility through our cold-chain center. Everything is research use only, not for human or veterinary use.
Yes. Every lot is independently tested before it can be sold. Peptriva carries research-use-only peptides, and the live catalog page is the only accurate list of what is in stock.
Every lot is tested twice by third-party labs. Once when the lot is finished, and again when it reaches our cold-chain center. The independent laboratory, currently Freedom Diagnostics in Franklin, TN, confirms identity by LC-MS, measures purity by HPLC-UV, weighs net content in mg, and runs a USP <85> endotoxin assay in duplicate. Heavy metals are tested within the purity panel.
This is enforced in code, not policy. Our system refuses to move a lot into sellable stock unless both the manufacturing facility certificate and the independent certificate are on file. Everything is research use only, not for human or veterinary use.
Yes. Certificates are issued per lot, not per order, so the document for a past order is the certificate for the lot you received. Give us your order number or the lot number and we will send the certificates on file for that lot.
Those records persist. A lot cannot enter sellable stock at all until both the manufacturing-facility certificate and the independent laboratory certificate are on file, and that check is enforced in our system, not by policy. The independent certificate prints identity by LC-MS, purity by HPLC-UV area-%, measured net content in mg, and endotoxin by LAL assay in accordance with USP <85>.
All products are research use only. Not for human or veterinary use.