The certificate behind every lot — searchable by batch.

A certificate of analysis (COA) is a laboratory report for one specific lot. It records which tests were run, the method used for each, and the result measured on material drawn from that lot. It is evidence about a batch, not a general statement about a compound: a COA for one lot says nothing about any other lot, which is why every lot here carries its own, identified by lot number and laboratory accession number.

Purity here is HPLC-UV area-percent, run by reversed-phase HPLC to USP <621>. The instrument separates everything in the sample and measures how much ultraviolet light each component absorbs; the purity figure is the target peptide’s share of the total absorbance. A result of 99.6% means the target peptide accounts for 99.6% of what the detector saw. The remainder is typically truncated sequences and synthesis by-products. Peptriva releases at 99% or higher, measured per lot rather than on a representative sample.

By liquid chromatography–mass spectrometry (LC-MS), to USP <736>. Every peptide sequence has one exact monoisotopic mass predicted by its formula. The mass spectrometer measures the mass of what is actually in the vial and matches it against that theoretical value to within 5 parts per million. A mislabelled vial, a truncated chain or a substituted analogue all fail this test, because the measured mass no longer matches the sequence on the label. Purity and identity answer different questions: identity asks whether it is the right molecule, purity asks how much of the sample is that molecule.

Endotoxins are fragments of the outer membrane of Gram-negative bacteria. They survive processes that kill the bacteria themselves, so material can carry them even when nothing is growing in it. They are measured by the Limulus amebocyte lysate (LAL) assay in accordance with USP <85>, run in duplicate. The certificate reports the outcome of that assay as a result rather than as a concentration, so read it as a pass on the method named. It is reported because endotoxin is a known confounder in sensitive in-vitro work: it can provoke a cellular response that is mistaken for an effect of the compound being studied.

Three contamination screens are run on the powder itself. Endotoxin by LAL, as above. Microbial contamination by PCR, which looks for bacterial and fungal DNA rather than waiting to see whether anything grows. Heavy metals by ICP-MS, against the elemental impurity limits in ICH Q3D. Together these describe what is in the vial besides the peptide.

Net content is the mass the laboratory actually weighed in the vial, not the nominal figure printed on the label. A vial labelled 10 mg reporting 10.17 mg contains slightly more than its label states. Reporting the measured mass rather than the nominal one lets you confirm the vial holds at least the amount you ordered, and lets you use the real figure in your own calculations instead of assuming the label.

Every lot is tested twice. The manufacturing facility tests and releases the batch, and that certificate is kept on file. The lot is then re-tested on arrival by Freedom Diagnostics of Franklin, Tennessee, an independent ISO/IEC 17025-accredited laboratory that neither manufactures nor sells the material. A lot cannot move into sellable stock until both certificates are on file, and if the two disagree the lot is not sold. The point of the second test is that the party selling the material does not control the only document verifying it.

ISO/IEC 17025 is the international standard for the competence of testing laboratories. An accredited laboratory has been assessed by a recognised accreditation body on its methods, equipment calibration, staff competence and quality system, and is re-assessed periodically. It accredits the LABORATORY, not the product: it means the result was produced by a lab judged competent to run that method. It is not a product certification and it is not an approval of any kind.

A COA reports measurements, and only the measurements it lists. It does not establish that a compound is safe, effective or approved for any use, and no result on it should be read that way. These are lyophilized powders in sealed, lot-coded vials, not sterile-filled preparations, so no sterility claim is made; what is reported instead is the endotoxin and microbial screening above. A certificate also describes the lot as the laboratory received it, so storage and handling after that point are outside what it can speak to. All products are for in-vitro laboratory research only. Not for human or veterinary use.

Match the lot number printed on the vial to the lot number on the certificate. Each report also carries the laboratory’s own accession number for the sample it received, which ties the result to a specific submission rather than to a reusable file. Search this page by lot or by compound to pull the record for the vial in front of you.